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The production superoxide anion was assayed as the difference between total and SOD-inhibitable cytochrome c reduction. 50 μg of total mitochondrial enriched-fraction protein treated or not with the 980 nm laser was added to 100 μM cytochrome c and 300 U SOD, if present, and the changes in absorbance of reduced cytochrome c was measured spectrophotometrically, at 550 nm [19].
Copyright and License information: ©2021 Andrea Amaroli et al.
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