mEPSC recordings

LT Lauren Tereshko
YG Ya Gao
BC Brian A Cary
GT Gina G Turrigiano
PS Piali Sengupta
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Whole cell voltage clamp recordings were obtained from neurons held at −70 mV. AMPAR-mediated currents were isolated by adding 25 μM picrotoxin, 25 μM APV, and 0.1 μM TTX to aCSF. Events that were <5 pA in amplitude or <3 ms in rise time were excluded from analysis. Recordings were analyzed as described using custom scripts in IGOR Pro (Joseph and Turrigiano, 2017; Tatavarty et al., 2020) (https://github.com/latereshko/mEPSC_scripts; Tereshko, 2021b; copy archived at swh:1:rev:4f77b23d5f94e6e8c5e1a76a059cae4f1811659a).

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