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Subcellular Localization

YT Yuehui Tang
KL Kun Liu
JZ Ju Zhang
XL Xiaoli Li
KX Kedong Xu
YZ Yi Zhang
JQ Jing Qi
DY Deshui Yu
JW Jian Wang
CL Chengwei Li
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The full length coding domain sequence of JcDREB2 (after removal of the termination codon) was amplified by RT-PCR from total RNA extracted from physic nut leaves using the primers given in Supplementary Table S1. The target sequence was cloned into the pSAT6-eYFP-N1 vector to construct the pSAT6-JcDREB2-eYFP fusion expression vector. The 35S::JcDREB2-YFP construct and a control (35S::YFP) plasmid were introduced into Arabidopsis protoplasts by the polyethylene glycol (PEG) mediated method. Next the transformed cells were incubated in the light for 16 h at 25°C. YFP fluorescence signal was detected by laser scanning confocal microscopy. Arabidopsis protoplasts was prepared according to Axelos (1992).

Copyright and License information:  ©2017 Tang, Liu, Zhang, Li, Xu, Zhang, Qi, Yu, Wang and Li.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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