Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Highly-multiplexed CodePlex chip secretome proteomics

PS Peter A. Szabo
PD Pranay Dogra
JG Joshua I. Gray
SW Steven B. Wells
TC Thomas J. Connors
SW Stuart P. Weisberg
IK Izabela Krupska
RM Rei Matsumoto
MP Maya M.L. Poon
EI Emma Idzikowski
SM Sinead E. Morris
CP Chloé Pasin
AY Andrew J. Yates
AK Amy Ku
MC Michael Chait
JD Julia Davis-Porada
XG Xinzheng V. Guo
JZ Jing Zhou
MS Matthew Steinle
SM Sean Mackay
AS Anjali Saqi
MB Matthew R. Baldwin
PS Peter A. Sims
DF Donna L. Farber
request Request a Protocol
ask Ask a question
Favorite

Cryopreserved tracheal washes and plasma were thawed at room temperature for 30-60 minutes and mixed well by pipetting up and down prior to loading. An aliquot of 5.5 μL of each sample was pipetted into each macrochambers of a CodePlex chip pre-patterned with a complete copy of a 23-plex antibody array. 2% BSA/PBS was used as background control. The chip was then loaded into an IsoLight automation system and various proteins were measured by fluorescence ELISA and analyzed by the IsoSpeak software using the IsoPlexis Human Adaptive Immune Panel: GM-CSF, Granzyme B, IFN-γ, IL-10, IL-13, IL-15, IL-17A, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IP-10, MCP-1, MIP-1α, MIP-1β, Perforin, sCD137, TGF-β1, TNF-α, TNF-β.

Copyright and License information:  ©2021 Elsevier Inc.
Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A