DEGs were identified using the limma package [37]. The absolute value of | log2 fold change (FC) | was set to >1, and the cutoff value was adjusted to a P-value of < 0.05, which was considered to be statistically significant. The FDR and P values were screened to obtain all DEGs. The ggplot2 and the heatmap package were used to plot volcano diagrams and heatmap of DEGs, respectively.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.