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Cells were stained using the Click-iT EdU Flow Cytometry Assay Kit (Invitrogen #C10424), and FxCycle Violet Stain (Invitrogen #F10347). Treated cells were incubated with 10 μM EdU for 24 hours. U2OS cells were the positive control. NOKs not treated with EdU were the negative control. Cells were analyzed on a Becton-Dickinson LSRII flow cytometer. Results were plotted using FlowJo v10 software (FlowJo LLC).
Copyright and License information: ©2021 Schwartz et al.
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