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EdU and FxCycle violet

RS Rachael E. Schwartz
MS Maxim N. Shokhirev
LA Leonardo R. Andrade
JG J. Silvio Gutkind
RI Ramiro Iglesias-Bartolome
GS Gerald S. Shadel
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Cells were stained using the Click-iT EdU Flow Cytometry Assay Kit (Invitrogen #C10424), and FxCycle Violet Stain (Invitrogen #F10347). Treated cells were incubated with 10 μM EdU for 24 hours. U2OS cells were the positive control. NOKs not treated with EdU were the negative control. Cells were analyzed on a Becton-Dickinson LSRII flow cytometer. Results were plotted using FlowJo v10 software (FlowJo LLC).

Copyright and License information:  ©2021 Schwartz et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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