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Concentration and Size Distribution

CG Corina A. Ghebes
JM Jess Morhayim
MK Marion Kleijer
MK Merve Koroglu
SE Stefan J. Erkeland
RH Remco Hoogenboezem
EB Eric Bindels
FA Floris P. J. van Alphen
MB Maartje van den Biggelaar
MN Martijn A. Nolte
BE Bram C. J. van der Eerden
EB Eric Braakman
CV Carlijn Voermans
JP Jeroen van de Peppel
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Concentration and size distribution of BMSC-derived EVs were determined using NanoSight NS300 (Nanosight Ltd., United Kingdom) equipped with a 405 nm laser. The particles in each sample were recorded for 5 times 60 s. The data was processed by NTA 2.3 software.

UCB-CD34+ cells, before and after 10 days’ culture, were examined for surface markers and viability. Absolute numbers were determined using fluorescence reference counting beads (Thermo Fisher Scientific, United States), anti-APC-CD34+ (Biolegend, United Kingdom) or anti-PeCy7-CD34+ (BD Bioscience, United States), anti-FITC-CD45 (BD Bioscience, United States), anti-APC-CD38 (eBioscience, Thermo Fisher Scientific, United States), anti-PE-CD45RA (Diaclone, France), and DAPI (Sigma Aldrich, Germany). All samples were analyzed using LRS II (BD Biosciences, United States) and data was analyzed using FlowJo software (Tree Star, Inc., United States).

Copyright and License information:  ©2021 Ghebes, Morhayim, Kleijer, Koroglu, Erkeland, Hoogenboezem, Bindels, van Alphen, van den Biggelaar, Nolte, van der Eerden, Braakman, Voermans and van de Peppel.
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