Plasma levels of GLP-1 measurement

IC I-Wen Chou
YC Yu-Hong Cheng
YC Yet-Ran Chen
PH Patrick Ching-Ho Hsieh
KK Klim King
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Blood samples were collected from the tail vein before any drug administration and at 15 and 60 min after i.p. glucose loading (time 0), as well as added DPP4i (10 μL per milliliter of blood) by following the manufacturer’s protocols. Plasma GLP-1 levels were determined by a high sensitivity GLP-1 active chemiluminescent 96-Well plate assay (EZGLPHS-35K, Merck Millipore).

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