Blood samples were collected from the tail vein before any drug administration and at 15 and 60 min after i.p. glucose loading (time 0), as well as added DPP4i (10 μL per milliliter of blood) by following the manufacturer’s protocols. Plasma GLP-1 levels were determined by a high sensitivity GLP-1 active chemiluminescent 96-Well plate assay (EZGLPHS-35K, Merck Millipore).
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.