2.2. Culture of AC16 human cardiomyocyte cell line

AC 16 cells (#SCC109; MilliporeSigma) were incubated in medium Dulbecco's modified Eagle's medium/Nutrient Mixture F‐12 Ham (DMEM/F‐12) (#D6434; MilliporeSigma) containing 2 mmol/L L‐glutamine, 12.5% fetal bovine serum (FBS), and 1X penicillin‐streptomycin (#TMS‐002‐C, ES‐009‐B, and TMS‐AB2‐C; MilliporeSigma) at 37°C in a humidified incubator with 5% CO₂. The medium was exchanged for fresh medium every 2‐3 days. After reaching to 90% confluency, cells were dissociated with trypsin‐EDTA (#SM‐2003‐C; MilliporeSigma) for further passage or experiments. In some experiments, cells were exposed to LPS and/or TB‐peptide in the conditions described in figures.