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Sample preparation and nLC-MS/MS analysis

MR Miguel Angel Merlos Rodrigo
HM Hana Michalkova
VS Vladislav Strmiska
BC Berta Casar
PC Piero Crespo
VR Vivian de los Rios
JC J. Ignacio Casal
YH Yazan Haddad
RG Roman Guran
TE Tomas Eckschlager
PP Petra Pokorna
ZH Zbynek Heger
VA Vojtech Adam
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Protein extraction and processing and proteomic profiling using nLC-MS/MS was carried out according to Merlos Rodrigo et al.46. The MS analyses were carried out using Q-Exactive mass spectrometer (Thermo Fisher Scientific) utilizing 2000 V of liquid junction voltage for sample ionization and capillary temperature of 270 °C. Mass spectrometer was coupled to Easy-nLC 1000 nano system (Thermo Fisher Scientific). The protein identification by nLC-MS/MS was carried out in the Proteomics and Genomics Facility (CIB-CSIC), a member of ProteoRed-ISCIII network.

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