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Plaque reduction assay

HZ Hanjun Zhao
KT Kelvin K. W. To
HL Hoiyan Lam
XZ Xinxin Zhou
JC Jasper Fuk-Woo Chan
ZP Zheng Peng
AL Andrew C. Y. Lee
JC Jianpiao Cai
WC Wan-Mui Chan
JI Jonathan Daniel Ip
CC Chris Chung-Sing Chan
MY Man Lung Yeung
AZ Anna Jinxia Zhang
AC Allen Wing Ho Chu
SJ Shibo Jiang
KY Kwok-Yung Yuen
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Peptides (P9R, P9RS and 8P9R) were synthesized by ChinaPeptide. Antiviral activity of peptides was measured using a plaque reduction assay. Briefly, peptides were dissolved in PBS or 30 mM PB containing 24.6 mM Na2HPO4 and 5.6 mM KH2PO4 at a pH of 7.4. Peptides or bovine serum albumin (BSA, 0.2–25.0 μg ml−1) were premixed with 50 PFU of coronavirus (SARS-CoV-2) in PBS or PB at room temperature. After 45–60 min of incubation, peptide-virus mixture was transferred to Vero-E6 cells, correspondingly. At 1 h post infection, infectious media were removed and 1% low melting agar was added to cells. Cells were fixed using 4% formalin at 3 day post infection. Crystal blue (0.1%) was added for staining, and the number of plaques was counted.

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