2.3. Two Kidneys-One Clip (2K1C) Hypertension

Simultaneously with ovarian resection, 21 animals among 44 were subjected to implement 2K1C by an incision on the right side of the abdomen, the right renal artery was isolated, and a U-shaped silver clip (lumen diameter of 0.2 mm) was placed around the artery to induce partial occlusion [17]. The other 23 ovariectomized animals also had a complete surgical intervention, just without a silver clip around the artery. These animals were considered control or normotensive (non-2K1C). After four weeks, we used the left kidney (nonclipped) for the measurements.