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Pollen grains were collected and incubated in GUS staining solution (0.05 mM PBS, pH7.0) with 1 mM 5-bromo-4-chloro-3-indolyl-β-D-Glucopyranosiduronic acid (X-gluc) at 37°C for 15 mins. Then the stained pollen samples were directly mounted on a glass slide for examination under either a Zeiss Axioskop microscope or a Nikon Eclipse Ti microscope. For DAPI staining, pollen tetrads from varies flower stages were released into DAPI solution on a glass slide and observed with a Zeiss Axioskop fluorescence microscope.
Copyright and License information: ©2016 Cilano et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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