Blood Pressure Measurement Using Telemetry Transmitter Implantation

Radio-telemetry probes were used to measure blood pressure in freely moving mice as described before (Grobe et al., 2015). Briefly, under isoflurane anesthesia, an incision was made on the neck and a sterile catheter of the telemetry transducer/transmitter (model TA11PA-C10, Data Sciences International, St Paul, MN, United States) was inserted into the carotid artery and secured with 5.0-gauge silk suture (Arosurgical, Newport Beach, CA, United States). The body of the transmitter device was placed in a subcutaneous sack on the animal’s flank. A suture was used to close the skin and the wound was cleaned with betaine solution. Subcutaneous Carprofen (5 mg/kg, Sigma-Aldrich, St. Louis, MO, United States) was administered immediately after surgery, and 24 h later for post-operative analgesia. Blood pressure and locomotor activity were measured 1 week after telemetry surgery, 2 days before the 2K1C surgery, second, and three weeks after 2K1C surgery.