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Brain samples were placed at room temperature for 1 h after storage at 4°C for 12 h. PBS (10 times the volume of brain tissue) was then added for homogenization. The supernatant samples were collected after centrifugation (5,000 rpm, 15 min, room temperature) and the activity of PFK and KHK enzymes was measured immediately using enzyme-linked immunoassay (ELISA) kits (PFK kit, Kete, Yancheng, China and KHK kit, Meimian, Yancheng, China), according to the manufacturers’ instructions, with six biological replicates per treatment.
Copyright and License information: ©2021 Lin, Fan, Han, Guo, Hao, Cao, Kang, Wang, He and Li.
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