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For luciferase reporter assay, PC3 cells (2 × 105 cells per well of six-well plate) were transfected with pGL3-KLK3-Luc reporter plasmid or the control plasmid along with AR-FL or AR-V7. The luciferase activity was determined 24 to 48 h after transfection using a dual luciferase reporter assay system following the manufacturer’s instructions (Promega, Madison, WI, USA), the signal was normalized to Renilla luciferase control as relative luciferase units.
Copyright and License information: ©2021 Liu, Liu, Yan, Liu, Fang and Fan
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