EdU (5-ethynyl-2-deoxyuridine) cell proliferation assay

SJ Shuang Jiang
RS Rui Sheng
XQ Xingying Qi
JW Jun Wang
YG Yuchen Guo
QY Quan Yuan
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Cells on cover glass were incubated with odontogenic medium containing 50 μmol·L−1 EdU for 8 h and were fixed with 4% PFA for 20 min. EdU was labeled with Click-iT EdU Imaging Kit (Invitrogen). Then sections were further incubated with DAPI to strain nuclei and imaged by laser scanning confocal microscopy (LSCM; Olympus FV3000).

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