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N2A cells were cultured in DMEM supplemented with 1× FCS, 1× penicillin–streptomycin, and 10 mM HEPES. When the N2A culture reached ~70% confluency, the cells were trypsinized and the cell suspension was centrifuged. The cells were seeded in 1.5 × 105 cells/well onto 6-well plate.
Copyright and License information: ©2021, Tapias et al
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