IncuCyte cell confluence measurements

ARN8 or HCT116 p53 WT or KO cells were seeded in a 96-well plate at a density of 4000 cells/well in a volume of 100 μl. The cells were incubated as described above for about 24 h prior to treatment. The medium of the assigned wells was replaced with 100 μl of fresh medium containing a 5 μm concentration of the corresponding Tenovins or DMSO control and placed in an IncuCyte S3 system located inside a CO₂ incubator. The cell growth was monitored every 2 h for a total of 72 h after an initial 30-min incubation period. The cell confluence percentage was analyzed by IncuCyte S3 2019 Rev 1 software (Essen Biosciences).