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Protein analysis of the SC
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Protein contents in the SC of the RHEK models were used for correction of ceramide contents. Precipitates remaining after lipid extraction were dissolved in a mixture of 10% sodium dodecyl sulfate (SDS) and 1N NaOH (1:9) at 60°C for 2 h. The mixtures were then neutralized with 2N HCl and the total protein amounts were determined by the BCA method.
Copyright and License information: ©2021 Takeda et al
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