This section was performed as already described [22]. For the wound healing assay, 4 × 105 cells were plated onto a 6-well plate. Twenty-four hours later, a strip of cells was removed from the monolayer of cells using a pipette tip. Phase contrast images were acquired with a 10× objective at the time of the scratch and 10 h later using a Nikon Eclipse TS100 microscope. Quantification was done using Image J. Data at 10 h were normalized to the size of the wound at 0 h and results were plotted using GraphPad (La Jolla, CA, USA) software.
Statistical analysis were performed with GraphPad (La Jolla, CA, USA) software.
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