2.7. Extraction of polyphenols

Thirty milligrams of lyophilized plant were homogenized with 0.75 mL of acidified methanol (HCl 0.1 % v/v), samples were subsequently placed in an ultrasonic bath for 15 min, and then centrifuged at 12.500 rpm for 5 min, and the supernatant was separated from the sediment. Finally, supernatants were pooled and stored at -80 °C until analysis. The entire procedure was carried out in darkness, and the extractions were carried out in triplicate.