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Analysis of exosomes by immunoblotting was performed using standard protocols: proteins were denatured, separated on 4–12% polyacrylamide gels, transferred onto a nitrocellulose membrane and probed with antibodies against tetraspanins CD9 (Santa Cruz Biotechnology) and CD63 (Santa Cruz Biotechnology). The immunocomplexes were visualized by chemiluminescence using the Chemidoc MP imaging system (Bio-Rad Laboratories). Signal intensity of the bands was measured by using Image Lab software (Bio-Rad Laboratories).
Copyright and License information: ©2021 Barberis, Vanella, Falasca, Caneapero, Cappellano, Raineri, Ghirimoldi, De Giorgis, Puricelli, Vaschetto, Sainaghi, Bruno, Sica, Dianzani, Rolla, Chiocchetti, Cantaluppi, Baldanzi, Marengo and Manfredi.
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