Transepithelial-Electric Resistance (TEER) Measurements

DH David Holthaus
ED Estefanía Delgado-Betancourt
TA Toni Aebischer
FS Frank Seeber
CK Christian Klotz
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To evaluate the barrier integrity and monolayer formation, TEER measurements were conducted. For this, a Millicell ERS-2 Voltohmmeter and a chopstick Ag/AgCl electrode (STX01) were used. For normalization, blank electric resistance (cell-free, coated transwell insert) was subtracted from raw resistance values and standardized for 1 cm² surface area. All measurements were conducted on a preheated 37°C heating block. For TEER measurements 12 to 36 filter inserts per species were analyzed.

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