To evaluate the barrier integrity and monolayer formation, TEER measurements were conducted. For this, a Millicell ERS-2 Voltohmmeter and a chopstick Ag/AgCl electrode (STX01) were used. For normalization, blank electric resistance (cell-free, coated transwell insert) was subtracted from raw resistance values and standardized for 1 cm² surface area. All measurements were conducted on a preheated 37°C heating block. For TEER measurements 12 to 36 filter inserts per species were analyzed.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.