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The treated cells were collected and resuspended in staining buffer, then incubated with FLICA 660-YVAD-FMK (FLICA 660 Caspase-1 Assay, Catalog number: 9122) and propidium iodide (PI, Thermo Fisher) for 25 min at 4°C. The ratios of activates caspase-1 and PI-positive cells were analyzed by flow cytometry (BD Biosciences).
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