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Multi-Locus Sequence Typing (MLST)

SI Sana Ilyas
MR Muhammad Hidayat Rasool
MA Muhammad Javed Arshed
MQ Muhammad Usman Qamar
BA Bilal Aslam
AA Ahmad Almatroudi
MK Mohsin Khurshid
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For the MLST, seven housekeeping genes (adk, fumC, gyrB, icd, mdh, purA, and recA) were amplified and sequenced according to the conditions provided by the EnteroBase Database.24 The obtained PCR products were sent for sequencing to Macrogen (South Korea). The raw sequences were edited using the ChromasPro software (Technelysium, Australia). The sequences were aligned using the ClustalW algorithm (MEGA7 software). The allele number was assigned to each gene locus and the sequence types (STs) were determined according to the allelic profiles for each of the isolates.

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