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Mouse striatal cells were plated at 60–70% confluency, on the next day were incubated with vehicle (DMSO) or harringtonine (2 μg/ml final concentration) for indicated timepoints or puromycin (100 μg/ml) for 20 min at 37 °C. The cells were immediately incubated with CHX (100 μg/ml) for 10 min and then scraped. Polysome profiles for each sample were collected and area under the curve for PS and 80S (MS) peaks in control and HD-homo cells, using PeakChart (v. 2.08, BRANDEL), and expressed as a ratio of PS/MS (Supplementary Fig. S1).
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