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Kinesore + QPD-OTf treatment in live cells
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U2OS or PTK2-GFP-Tubulin cells (1.5 × 105) were seeded into 3.5-cm-glass bottom dishes with 10 mm microwell (Mattek); cells were incubated in a culture medium at 37 °C under 5% CO2 in a humidified incubator for 24 h. Then media was removed, cells were washed twice with DMEM (−) (no additives), Kinesore (100 µM) + QPD-OTf (20 µM) were then added to cells in Ringer’s buffer and cells were incubated at 37 °C under 0% CO2 for (1.5 h for PTK2; 2 h for U2OS). Cells were imaged with a LEICA SP8.
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