Probes of fluorescent RAB8A and paxillin were expressed in astrocytes via lentiviral transduction. FIV particles were produced in the HEK293TN producer cell line (BioCat) and harvested analogously to HIV particles77. In all, 70% confluent astrocytes from WT and Dbn−/− mice on glass-bottom dishes were transduced 7 days before scratch wounding and live-cell imaging.
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