2.8. Cell cycle assay

The distribution of the cell cycle was evaluated using a cell cycle analysis kit (Beyotime, C1052) in accordance with the instruction. Briefly, SGC7901 cells (2*10⁵ cells/well) were placed in 6‐well plates. After 24 h, the cells were collected using trypsin digestion and centrifugation. After washing with cold PBS, cells were fixed in 70% methanol at 4°C overnight. Then, the cells were stained with a propidium iodide (PI) that containing 10 mL of RNase A at 37°C for 30 min. The samples were analyzed using a FACS flow cytometer (BD C6) and at least 10 000 cells were collected per sample. The data were analyzed using FlowJo 10.0 software (TreeStar).