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Isolation and Detection of Treponemal DNA

DL Dan Liu
SH Shu-Min He
XZ Xiao-Zhen Zhu
LL Li-Li Liu
LL Li-Rong Lin
JN Jian-Jun Niu
TY Tian-Ci Yang
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DNA was isolated from the patient’s lesion swabs or CSF using a QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) in accordance with the manufacturer’s protocol (Tong et al., 2017; Zhang et al., 2020). Real-time PCR targeting tp0574 was performed to determine the copies of treponemal DNA. The DNA samples that tested positive were stored at -20°C for the next procedure.

Copyright and License information:  ©2020 Liu, He, Zhu, Liu, Lin, Niu and Yang
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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