Indirect calorimetry experiments

PR Patricia René
DL Damien Lanfray
DR Denis Richard
MB Michel Bouvier
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Mice were placed in metabolic chambers (AccuScan Instruments, Inc.) and acclimated for 72 hours with free access to food and water. Then, VO2 and VCO2 were evaluated every 15 minutes over 24 hours in an open circuit system with an oxygen analyzer (Applied Electrochemistry, S-3A1) and carbon dioxide analyzer (Applied Electrochemistry, CD-3A) as previously described (46). Energy expenditure data are represented by AUC/lean mass as differences have been observed in lean mass between groups.

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