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The rotarod test was performed at 7 and 24 weeks of age to evaluate coordination and motor skill acquisition (type 7650; Ugo Basile). The mice were placed on the rotating rod (3 cm diameter, 30 cm long) for 4 trials every day for a period of 4 days. Each trial lasted a maximum of 10 minutes. The rod accelerated from 4 to 40 rpm in 5 minutes and remained at 40 rpm for the remaining 5 minutes. The time the mice took to fall was recorded. Two subsequent rotations around the rod were also counted as a fall.
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