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HSP90β ATPase activity was performed in the reactions (33 mM HEPES [pH7.2], 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1 μM ATP) with or without indicated compounds at 37°C for 1 hour; then, the CellTiter-Glo Assay was employed to measure the quantitation of ATP according to the manufacturer’s instructions, and luminescence was recorded.
Copyright and License information: ©2021 Xu et al.
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