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2.8. SARS-CoV-2 pseudovirus neutralization assay
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For pseudovirus-based neutralization assay, hACE2-293T cells were seeded in 96 well-plates 18 h before infection. Serum samples were serially diluted and co-cultured with the same volume of pseudotype particles at 37 °C for 1 h. The mixtures were duplicated and added to the monolayers of hACE2 expressing cells in 96-well microtiter plates. Then, the plates were incubated at 37 °C in 5% CO2. Forty-eight h later, the cells were lysed and measured using Promega Glomax (GloMax® Explorer). The results were presented as NT50.
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