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After 48h incubation with or without AZD2014 or rapamycin, cells were harvested and resuspended in fixation fluid. 5 µl Annexin V‐FIFC and 5µl propidium iodide were added to 500 µl cell suspension. Cell apoptosis was then determined by using FACSort flow cytometer (BD Bioscience). In the graphs, the quadrant, respectively, stands for dead cells, living cells, early apoptotic cells and late apoptotic cells.
Copyright and License information: ©2021 The Authors. published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd
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