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2.2.2. Effects of HFF/FW duration on adipokine expression in the POATs and serum

PY Po-Kai Yang
CC Chia-Hung Chou
CH Chu-Chun Huang
WW Wen-Fen Wen
HC Hsin-Fu Chen
CS Chia-Tung Shun
HH Hong-Nerng Ho
MC Mei-Jou Chen
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The effects of different durations of the HFF/FW diet on adipokine expression in the POATs were evaluated. The animals were again allocated into STD (N = 40) and HFF/FW (N = 40) diets at 6 weeks of age. After each completed week of dietary manipulation over a 4-week period, mice (N = 10/group/week) were randomly chosen from each treatment group and sacrificed under anesthesia with 5% isoflurane. Blood and ovaries were collected using the methods described in Section 2.2.1. The POATs were harvested and stored in liquid nitrogen for subsequent protein extraction and assay.

Copyright and License information:  ©2021 The Author(s)
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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