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Chiral HPLC analysis

BF Britta Frensch
TL Thorsten Lechtenberg
MK Michel Kather
ZY Zeynep Yunt
MB Martin Betschart
BK Bernd Kammerer
SL Steffen Lüdeke
MM Michael Müller
JP Jörn Piel
RT Robin Teufel
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Dissolved and filtered samples of lenticulone (11) were analyzed by chiral HPLC on a 1100 series chromatographic system (Agilent Technologies) using a CHIRALPAK® AS-H (250 × 4.6 mm ID, 5 µM, Chiral Technologies Europe). The mobile phase was 100% ethanol. The flow rate was 0.25 mL/min at 30 °C and absorbance was measured at 240 nm as well as 350 nm, and 495 nm. Additional tested columns: CHIRALPAK® AD-H (250 × 4.6 mm ID, 5 µM, Chiral Technologies Europe), CHIRALCEL® OD-H (250 × 4.6 mm ID, 5 µM, Chiral Technologies Europe) and MultoHigh Chiral-AM-HR (250 × 4.6 mm ID, 5 µM, Isera).

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