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Samples and Peptides

SP Sabrina Pollastro
MB Marie de Bourayne
GB Giulia Balzaretti
AJ Aldo Jongejan
BS Barbera D. C. van Schaik
IN Ilse T. G. Niewold
AK Antoine H. C. van Kampen
BM Bernard Maillère
NV Niek de Vries
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Blood samples were obtained from healthy donors at Etablissement Français du Sang (EFS), Rungis, France, after informed consent from the donor and in accordance with EFS guidelines approved by the Comité d’éthique et de déontologie de l’EFS. Peripheral blood mononuclear cells (PBMCs) were isolated from total blood using Ficoll/Lymphoprep separation (GE Healthcare) and cryopreserved in liquid nitrogen until further use. Peptides used for the T cell stimulation assay were selected from published CD4+ T cell epitopes from Cytomegalovirus, Epstein–Barr virus and Influenza A virus and synthesized by Pepscan (The Netherlands). Peptides sequences are reported in Supplementary Table S1.

Copyright and License information:  ©2021 Pollastro, de Bourayne, Balzaretti, Jongejan, van Schaik, Niewold, van Kampen, Maillère and de Vries
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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