Gut microbiota depletion

Gut microbiota was depleted in WT and Nlrp6^-/- mice with a broad-spectrum cocktail of antibiotics as previously described [51]. Initially, amphotericin B (0.1 mg/ml, every 12 hours) was administrated by oral gavage for 3 days, and subsequently, drinking water was supplemented with a cocktail composed of ampicillin (2 g/L), neomycin (2 g/L), metronidazole (1 g/L), and vancomycin (0.5 g/L) for 21 days. Additionally, all mice received ciprofloxacin i.p. (0.2 g/L). Feces were then collected and bacterial depletion were determinate on Thioglycolate medium, as described before [51]. Only mice with <1 CFU/mg feces were considered as depleted and used for further experiments.