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These assays measure the ability of test compounds to inhibit cytochrome P450 activity for four isoforms CYP1A2, CYP2C9, CYP2D6 and CYP3A4, with a time dependent inhibition component for 3A4 only, in human liver microsomes, using luminescent and/or fluorescent assay kits. The test compound’s ability to inhibit the enzyme activity (IC50) was assessed by determining the extent of inhibition of metabolite formation relative to vehicle control (DMSO).
Copyright and License information: ©2021 Ferreira et al
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