2.4. Western Blot

Immunoblots were obtained as we previously described and validated [28,30,31] using the Odyssey system (LI-COR Biosciences, Lincoln, NE, USA); the intensity of bands was quantified using the FIJI software.

The following antibodies were used: Neuropilin-1 (catalog number: #ST0530, Novus Biologicals, Bio-Techne, Minneapolis, MN, USA); Akt (catalog number: #sc-5298, Santa Cruz Biotechnology, Dallas, TX, USA); pAkt^Ser473 (catalog number: #9271, Cell Signaling, Danvers, MA, USA); β Actin (catalog number: #ab8229, abcam, Cambridge, MA, USA).