Cell Culture

SS Sandra Schneider
RC Rossella De Cegli
JN Jayapriya Nagarajan
VK Viola Kretschmer
PM Peter Andreas Matthiessen
DI Daniela Intartaglia
NH Nathan Hotaling
MU Marius Ueffing
KB Karsten Boldt
IC Ivan Conte
HM Helen Louise May-Simera
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ARPE-19 cells were kindly provided from Karsten Boldt (Tübingen) and cultured in DMEM/F-12 + GlutaMax (Gibco, #31331028) supplemented with 10% fetal bovine serum (FBS) (LONZA), and 1% penicillin/streptomycin (Thermo Fischer, #10378016) (referred to as complete medium). BBS8 siRNA (Dharmacon, #L-021417-02-0005) and non-targeting siRNA (IDT, TriFECTa) were used to generate BBS8 KD and Non-targeting control (NTC) cells. Transfections were performed using antibiotic-free medium in 96-well plates using Lipofectamine RNAiMAX reagent (Invitrogen, #113778150) according to manufacturer's recommendations for reverse transfections. Post transfection, the cells we cultured in complete medium. The phagocytosis assay was performed 48 h post-transfection.

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