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Whole-genome sequencing

LC Lars Custers
EK Eleonora Khabirova
TC Tim H. H. Coorens
TO Thomas R. W. Oliver
CC Camilla Calandrini
MY Matthew D. Young
FB Felipe A. Vieira Braga
PE Peter Ellis
LM Lira Mamanova
HS Heidi Segers
AM Arie Maat
MK Marcel Kool
EH Eelco W. Hoving
MH Marry M. van den Heuvel-Eibrink
JN James Nicholson
KS Karin Straathof
LH Liz Hook
RK Ronald R. de Krijger
CT Claire Trayers
KA Kieren Allinson
SB Sam Behjati
JD Jarno Drost
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DNA was extracted from either formalin-fixed paraffin-embedded (FFPE) tissues or fresh frozen tumour or tissue samples. Short insert (500 bp) genomic libraries were constructed and 75 base pair (FFPE) or 150 base pair (frozen) paired-end sequencing clusters were generated on either the Illumina HiSeq X or the Illumina NovaSeq platform according to Illumina no-PCR library protocols. An overview of samples and sequencing variables, including the average sequence coverage, is shown in Supplementary Table 1.

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