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At 28 days after CCI, the animals were perfused with 0.1 mol/L PBS under deep anesthesia. Brains were frozen-sectioned at the thickness of 10 μm. Brain slices 500 μm apart were stained with hematoxylin-eosin then photographed. The target level was between 1 mm anterior and 3 mm posterior to the bregma. Brain slices 500 μm apart (total nine sections in each animal) were collected to cover the core lesion area. The volume of injured tissue was measured with ImageJ 1.48 software. Damaged tissue volume = contralateral hemisphere volume−ipsilateral hemisphere volume.
Copyright and License information: ©2021 Liu, He, Wu, Du, Jiang, Chen, Yu, Zhong, Wang, Cheng, Sun and Huang.
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