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The mice were conditioned to a round plexiglass container for at least 5 min. A 1-inch piece of laboratory tape was gently applied to the bottom centre of the mouse’s back. The mice were observed for 5 min, and the total number of responses to the tape was recorded. Biting or grabbing the tape or making an obvious “wet dog shake” movement to remove the tape from the back was considered a response.
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