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An overnight bacterial culture was diluted 1:100 in BHI media containing various concentrations of eriodictyol ranging from 32 to 256 μg/mL and grown with shaking at 37°C to an A600nm of 0.6. Then, 5 μL of the bacterial culture was added to 195 μL of BHI broth enriched with 1% glucose to form a biofilm at 37°C for 18 h. Determination and quantitative analysis of the biofilm were performed as described previously (Merritt et al., 2011).
Copyright and License information: ©2021 Wang, Li, Li, Jing, Jin, Yang, Yu, Wang, Wang and Wang.
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