The Distribution of Tumor Mutation Burden (TMB) and TMB Score

TMB was calculated as mutations per megabase (mut/Mb). Mutational signature analysis was performed for tumors with somatic mutation counts of at least 10. With the development of cancer genomics, mutation annotation format (MAF) was widely accepted and used to store the detected somatic variants. Maftools is an R package that is published in Bioconductor⁶, and is specially used to visualizethe information in MAF files. The distribution of tumor mutation burden (TMB) was conducted by Maftools R package (Supplementary Table 3), which generated four results, including the summary of TMB, waterfall of mutation genes, interaction of mutation genes, and TMB score (Supplementary Table 9). Furthermore, the association between the expression of top mutation genes and drug sensitivity was performed by Corrplot R package (version 0.84) with the Pearson correlation coefficient (r > 0.5, and p < 0.05) based on the corresponding data from CellMiner⁷.