Holder pasteurization (HoP) was performed at the Regional Human Milk Bank in Warsaw at the Holy Family Hospital on an automatic Human Milk Pasteurizer S90 Eco (Sterifeed, Medicare Colgate Ltd., Post Cross Business Park, Kentisbeare, Cullompton, Devon, UK). Fifty-milliliter milk samples were treated for 30 min at 62.5 °C according to the standard operating pasteurization protocol.
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