4.4. Percentage of Growth

To assess the effects of FLC on C. albicans growth in the presence of different carbon sources, we followed the protocol described by the Clinical and Laboratory Standards Institute (2008), 3rd ed. M27-A3 [49] with modifications. Briefly, 10-mg/mL stock solution of FLC was serially diluted in YNBG or YNBF media using 96-well sterile plates (Sarstedt, Nümbrecht, Germany). The various media compositions were then inoculated with C. albicans suspensions (final A₆₀₀ = 0.01 per well) and prepared in fresh YNBG or YNBF media from 24-h YNBG cultures. After 24 h of incubation at 28 °C, A₆₀₀ was measured using a ASYS UVM 340 microplate reader (Biogenet, Józefów, Poland). The percentage of growth of the C. albicans CAF2-1, DSY448, DSY653, DSY465, DSY654, and DSY1050 strains was determined by normalizing A₆₀₀ to that observed under conditions without FLC.